![]() Our membrane protein isolation and membrane protein extraction kits are optimized specifically for your needs to isolate high-quality protein samples essential to completing successful downstream applications. Reagents also have the added benefits of both lysing and solubilizing effects. The Thermo Scientific membrane protein extraction and membrane protein isolation reagents consist of optimized concentrations of detergents, buffers, salts, and reducing agents developed for particular species and types of cells. Reagent-based lysis methods do not require expensive, cumbersome equipment and protocols that are difficult to implement. Reagent-based lysis has replaced traditional physical lysis as the method of choice for membrane disruption and extraction of membrane proteins. Protease or phosphatase inhibitors recommended? Western blot, ELISA, mass spectrometry analysis IP, western blot, ELISA, amine reactive labeling Western blot, IP, protein purification, radio-ligand binding assays Lysis buffer is compatible with BCA and Rapid Gold BCA elution buffer is not compatible. Less than 20% contamination of cytosolic proteinsġ00 samples with 10 7 cells or 100 samples with 50–100 mg tissueĥ0 samples with 5 million cells or 25 samples of 20–40 mg tissue per kitĨ experiments, with two confluent 15 cm dishes or four T75 flasksīCA Protein Assays, detergent-compatible Bradford Less than 5% contamination of cytosolic proteins Maintains structural integrity as measured by immunoprecipitation and receptor ligand binding assaysĮnrichment compatible with western blotting and MS applicationsĮnrichment compatible with western blotting Integral and membrane associated proteins ![]() Mem-PER Plus Membrane Protein Extraction Kit GPCR Extraction and Stabilization ReagentĬell Surface Protein Biotinylation and Isolation Kit
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